Evaluation of Genetic Diversity of Local and Exotic Tomatoes using Morphological Traits and Simple Sequence Repeat Molecular Markers
DOI:
https://doi.org/10.51459/jostir.2025.1.Special-Issue.099Keywords:
Diversity, Molecular, Morphological, Genotypes, MarkersAbstract
This study investigated the genetic diversity among local, exotic tomato varieties, and their progenies, recognizing the crop's global importance and the necessity of germplasm knowledge for breeding and conservation. The research employed both morphological traits and simple sequence repeat molecular markers. Morphological data were analyzed using Principal Component Analysis and Single Linkage Cluster Analysis, while molecular data were processed with Numerical Taxonomy and Multivariate Analysis System Software. Results revealed significant genetic diversity among the tomato genotypes. However, inconsistencies were observed in genotype clustering when comparing morphological and molecular characterization, although some genotypes showed consistent grouping across both methods. The study concludes by recommending the combined application of morphological traits and molecular markers for comprehensive diversity studies, leveraging their complementary effects for more robust insights into tomato germplasm.
References
Journal Articles
Adeniji, O.T. et al. (2020) 'Phenotypic variability for horticultural and fruit quality attributes in plastic house grown tomato', Journal of Horticultural Science, 15(2), pp. 136–146.
Cao, X. et al. (2015) 'A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes', Genetic Molecular Resource, 14, pp. 1650–1661.
Fayeun, L.S. et al. (2018) 'Phenotypic traits diversity in fluted pumpkin (Telfairia occidentalis Hook. F)', International Journal of Plant and Soil Science, 24(2), pp. 1–11.
Fiorani, F. and Schurr, U. (2013) 'Future scenarios for plant phenotyping', Annual Review of Plant Biology, 64, pp. 267–291.
Giovannetti, M. et al. (2012) 'Nutraceutical value and safety of tomato fruits produced by mycorrhizal plants', British Journal of Nutrition, 107, pp. 242–251. doi: 10.1017/s000711451100290.
Herison, C. et al. (2018) 'Genetic diversity analysis in 27 tomato accessions using morphological and molecular markers', AGRIVITA Journal of Agricultural Science, 40(1), pp. 36–44. doi: 10.17503/agrivita.v40i1.726.
Ibitoye. (2020) 'Genetic resources', Genetic Resources, 1(2), pp. 1–11. doi: 10.1007/s10681-007-9440-4.
Korir, N.K. et al. (2014) 'Genetic diversity and relationships among different tomato varieties revealed by EST-SSR markers', Genetic Mol. Resource, 13, pp. 43–53.
Liu, J. et al. (2024) 'Genetic regulation of fruit shape in horticultural crops: A review', Horticulturae, 10(11), p. 1151. doi: 10.3390/horticulturae10111151.
Liu, Z.B. et al. (2019) 'Research advance of plant heterosis', Molecular Plant Breeding, 17, pp. 4127–4134.
Lupaşcu, G., Grigorcea, S. and Mihnea, N. (2015) 'The influence of the maternal factor on the effects of gene involved in the control of quantitative characters in tomato', Oltenia. Studii şi comunicări. Ştiinţele Naturii, 31(1), pp. 1454–6914.
Mazzucato, A. et al. (2008) 'Genetic diversity, structure and marker-trait associations in a collection of Italian tomato (Solanum lycopersicum L.) landraces', Theoretical Applied Genetics, 116, pp. 657–669. doi: 10.1007/s00122-007-0699-6.
Muojiama, S.O., Olasanmi, B. and Ukwu, U. (2020) 'Fruit setting in cassava (Manihot esculenta Crantz) varieties as influenced by genotype and maternal inheritance', AJAAR, 13(4), pp. 36–43. doi: 10.9734/ajaar/2020/v13i430112.
Naz, S. et al. (2013) 'Assessment of genetic diversity within germplasm accessions in tomato using morphological and molecular markers', The Journal of Animal and Plant Sciences, 23(4), pp. 1099–1106.
Nikoumanesh, K. et al. (2011) 'Morphological and molecular variability in some Iranian almond genotypes and related Prunus species and their potentials for rootstock breeding', Scientia Horticulturae, 129, pp. 108–118.
Odiyi, A.C. et al. (2014) 'Analysis of variation among fluted pumpkin (Telfairia occidentalis) accessions by factor analysis and principal component analysis', Canadian Journal of Plant Breeding, 2(2), pp. 80–86.
Poczai, P. et al. (2011) 'Genetic diversity assessment of bittersweet (Solanum dulcamara, Solanaceae) germplasm using conserved DNA-derived polymorphism and intron-targeting markers', Annals of Applied Biology, 159(1), pp. 141–153. doi: 10.1111/j.1744-7348.2011.00482.
Ruiz, J.J. and Garcia-Martinez, S. (2005) 'Genetic variability and relationship of closely related traditional cultivars of Spanish tomatoes using SRAP and SSR markers', Journal of the American Society for Horticultural Science, 130(1), pp. 88–31.
Saleem, M.Y. et al. (2013) 'Diallel analysis of yield and some yield components in tomato (Solanum lycopersicum L.)', Pakistan Journal of Botany, 45(4), pp. 1247–1250.
Salim, M.M.R. et al. (2020) 'Morphological characterization of tomato (Solanum lycopersicum L.) genotypes', Journal of the Saudi Society of Agricultural Sciences, pp. 233–240.
Solomon, B. et al. (2008) 'Assessment of genetic variation in tomato (Solanum lycopersicum L.) inbred lines using SSR molecular markers', Journal of Genetics and Genomics, 35, pp. 373–379.
Verma, S., Sharma, V. and Kumari, N. (2020) 'Microwave pretreatment of tomato seeds and fruit to enhance plant photosynthesis, nutritive quality and shelf life of fruit', Journal of Postharvest Biology and Technology, 159, p. 111015. doi: 10.1016/j.postharvbio.2019.111015.
Zhou, R. et al. (2015) 'Genetic diversity of cultivated and wild tomatoes revealed by morphological traits and SSR markers', Genetics and Molecular Research, 14(4), pp. 13868–13879. doi: 10.4238/2015.
Zhu, Y.Z. et al. (2018) 'Sensory and flavor characteristics of tomato juice from Garden Gem and Roma tomatoes with comparison to commercial tomato juice', Journal of Food Science, 83, pp. 153–161. doi: 10.1111/1750-3841.
Online Source / Database
FAOSTAT (Food and Agriculture Organization Corporate Statistical Database) (2019) Food and Agriculture Organization of the United Nations. Available at: http://www.fao.org/faostat/en/?#data.
Dissertation
Grigorcea, S. (2014) Influenţa factorului matern asupra rezistenţei la patogenii fungici şi elementelor de productivitate la tomate (Solanum lycopersicum). PhD dissertation. Institute of Genetics, Physiology and Plant Protection of the Academy of Sciences of Moldova.
Report / Monograph
International Plant Genetic Resources Institute (1996) Descriptors for tomato (Lycopersicon spp.). Available at: http://pdf.usaid.gov/pdf_docs/PNACH866. (Accessed: 25 August 2025).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Journal of Science, Technology and Innovation Research
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.