Efficient Decolorization of Textile Dye by Immobilized Thermotolerant Laccase from Bacillus subtilis CFB-09
DOI:
https://doi.org/10.51459/jostir.2025.1.Special-Issue.076Keywords:
Bacillus subtilis CFB-09, Dye-decolorization, Laccase, Methyl blue, Thermal stabilityAbstract
The widespread discharge of textile dyes into aquatic environments has led to a growing public health concern, as a result of their toxicity in water bodies. Hence, there is urgent need for sustainable dye removal strategies. Enzyme-based bioremediation has emerged as a promising eco-friendly approach, with laccase, a multicopper oxidase, gaining attention due to its ability to oxidize a wide range of phenolic compounds including lignin-mimicking dyes. Therefore, this study explores the degradation of methyl blue dye using laccase from Bacillus subtilis CFB-09. The physicochemical properties of free and immobilized laccase were characterized, and their decolorization efficiency was evaluated across varied dye concentration (25-200 mg/L). Results showed that free and immobilized laccase exhibited optimal activity at 30 °C, pH 5.0 and 40 °C, pH 6.0, respectively. Free laccase demonstrated high thermal stability by retaining over 80% residual activity up to 70 °C for 180 min of incubation, while immobilized laccase exhibited a gradual decline in activity yet retained over 50% residual activity at 30-70 °C for 90 min. Decolorization studies of methyl blue showed that both free and immobilized laccase achieved maximum decolorization at lower dye concentrations. Remarkably, at 125 mg/L, immobilized laccase exhibited slightly higher decolorization (81.28%) than free laccase (78%) after 120 h, indicating enhanced stability and prolonged activity, while at higher concentration (200 mg/L), both free and immobilized laccase showed reduced efficiency (~6.87%). These findings suggest that both free and immobilized laccase hold promise as cost-effective and eco-friendly solutions for mitigating the environmental and health hazards associated with textile dye pollution.
References
Al-Tohamy, R., Ali, S. S., Li, F., Okasha, K. M., Mahmoud, Y. A. G., Elsamahy, T., and Sun, J. (2022). A critical review on the treatment of dye-containing wastewater: Ecotoxicological and health concerns of textile dyes and possible remediation approaches for environmental safety. Ecotoxicology and Environmental Safety, 231, 113160.
Ardila-Leal, L. D., Poutou-Piñales, R. A., Pedroza-Rodríguez, A. M., and Quevedo-Hidalgo, B. E. (2021). A brief history of colour, the environmental impact of synthetic dyes and removal by using laccases. Molecules, 26(13), 3813.
Atiroğlu, V., Atiroğlu, A., Atiroğlu, A., Al-Hajri, A. S., and Özacar, M. (2024). Green immobilization: Enhancing enzyme stability and reusability on eco-friendly support. Food Chemistry, 448, 138978.
Bandounas, L., Wierckx, N. J., de Winde, J. H., and Ruijssenaars, H. J. (2011). Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential. Bmc Biotechnology, 11, 1-11.
Bié, J., Sepodes, B., Fernandes, P. C., and Ribeiro, M. H. (2022). Enzyme immobilization and co-immobilization: main framework, advances and some applications. Processes, 10(3), 494.
Cerciello, A., Del Gaudio, P., Granata, V., Sala, M., Aquino, R. P., and Russo, P. (2017). Synergistic effect of divalent cations in improving technological properties of cross-linked alginate beads. International Journal of Biological Macromolecules, 101, 100-106.
Chauhan, P. S., Goradia, B., and Saxena, A. (2017). Bacterial laccase: recent update on production, properties and industrial applications. 3 Biotech, 7(5), 323.
Cottone, G., Giuffrida, S., Bettati, S., Bruno, S., Campanini, B., Marchetti, M., and Ronda, L. (2019). More than a confinement:“Soft” and “hard” enzyme entrapment modulates biological catalyst function. Catalysts, 9(12), 1024.
Defaei, M., Taheri-Kafrani, A., Miroliaei, M., and Yaghmaei, P. (2018). Improvement of stability and reusability of α-amylase immobilized on naringin functionalized magnetic nanoparticles: A robust nanobiocatalyst. International Journal of Biological Macromolecules, 113, 354-360.
Dubey, N. C., and Tripathi, B. P. (2021). Nature inspired multienzyme immobilization: Strategies and concepts. ACS Applied Bio Materials, 4(2), 1077-1114.
Falade, A. O., Nwodo, U. U., Iweriebor, B. C., Green, E., Mabinya, L. V., and Okoh, A. I. (2017). Lignin peroxidase functionalities and prospective applications. MicrobiologyOpen, 6(1), e00394.
Islam, T., Repon, M. R., Islam, T., Sarwar, Z., and Rahman, M. M. (2023). Impact of textile dyes on health and ecosystem: a review of structure, causes, and potential solutions. Environmental Science and Pollution Research, 30(4), 9207-9242.
Niladevi, K. N., and Prema, P. (2008). Effect of inducers and process parameters on laccase production by Streptomyces psammoticus and its application in dye decolourization. Bioresource Technology, 99(11), 4583-4589.
Olajuyigbe, F. M., Adetuyi, O. Y., and Fatokun, C. O. (2019). Characterization of free and immobilized laccase from Cyberlindnera fabianii and application in degradation of bisphenol A. International Journal of Biological Macromolecules, 125, 856-864.
Olajuyigbe, F. M., Afere, F. P., Adetuyi, O. Y., and Fatokun, C. O. (2021). Decolorization of lignin-mimicking dyes by Stenotrophomonas sp. CFB-09: Enzyme activity, transformation dynamics and process optimization. Biocatalysis and Biotransformation, 40(5), 351-364.
Panwar, V., Lzaod, S., and Dutta, T. (2023). Thermostable bacterial laccase: catalytic properties and its application in biotransformation of emerging pollutants. ACS omega, 8(38), 34710-34719.
Peter, J. K., and Kumar, P. V. (2014). Production optimization and partial purification of laccases from bacterial consortium. International Journal of Engineering Resources 3, 458-465.
Rajendran, S., Kalairaj, A., and Senthilvelan, T. J. B. C. (2025). A comprehensive review on enzymatic decolorization of various azo dyes using laccase for the abatement of industrial pollution. Biomass Conversion and Biorefinery, 15(9), 13079-13101.
Ramamurthy, K., Priya, P. S., Murugan, R., and Arockiaraj, J. (2024). Hues of risk: investigating genotoxicity and environmental impacts of azo textile dyes. Environmental Science and Pollution Research, 31(23), 33190-33211.
Ranimol, G., Paul, C., and Sunkar, S. (2021). Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1. Water Science and Technology, 84(10-11), 2703-2717.
Reinhammar, B. (2018). Laccase. In Copper proteins and copper enzymes (pp. 1-36). CRC press.
Zhang, W., Zhang, Z., Ji, L., Lu, Z., Liu, R., Nian, B., and Hu, Y. (2023). Laccase immobilized on nanocomposites for wastewater pollutants degradation: current status and future prospects. Bioprocess and Biosystems Engineering, 46(11), 1513-1531.
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