The Evaluation of the Solvent Fractions of the Fruits, Leaves, and the Stem Bark of Sarcocephalus latifolius (SM.) E. A. Bruce for Cytotoxicity and Antioxidant Activities
DOI:
https://doi.org/10.51459/jostir.2025.1.Special-Issue.066Abstract
The geared trend in using medicinal plants in the management of various human ailments, cancer inclusive has gained significant consideration. Despite the innovations in technology, and the advent of foremost therapies, there is a growing inclination towards herbal medicine. In traditional medicine, Sarcocephalus latifolius (SL) is utilized to treat cancer; however, its efficacy in doing so has not been scientifically confirmed or supported by empirical data. The study evaluated the antioxidant and the cytotoxicity profile of the fruits, leaves and the stem bark of SL. These plant parts were harvested, washed, air-dried, pulverised and soaked in 100% methanol to obtain the crude methanol extracts (CMESL, MCESLL and CMESBSL). These were partitioned successively between n-hexane chloroform, ethyl acetate and methanol to obtain their respective fractions, N-hexane (HFSL, HFSLL and HFSBSL), chloroform (CFSL, CFSLL, and CFSBSL), ethyl acetate (EFSL, EFSLL, and EFSBSL), and the methanol (MFSL, MFSLL and MFSBSL). These extracts and fractions were subjected to in vitro antioxidant assays (DPPH, TAC, TPC and FRAP) and an in vivo cytotoxicity test using the brine shrimp lethality test (BSLT), to determine their LC50. The results reveal that CFSL, CFSLL and CFSBSL is the most cytotoxic when compared to the other fractions, and for the antioxidant assays, the ethyl acetate fractions of the leaves and stem bark, EFSLL, and EFSBSL and the chloroform fraction, CFSL of the fruits, had the peak activity. Sarcocephalus latifolius plant demonstrates the potential as a rich reservoir for antioxidants and might have bioactive agents responsible for the anticancer effects.
References
Abbah, J., Amos, S., Chindoc, B., Ngazalc, I., Vongtaue, H.O., Adzuc, B., Faridad, T., Odutolad, A.A.,Wambebec, C., and Gamaniel, K.S. (2010). Pharmacological evidence favouring the use of Nauclea latifolia in malaria ethnopharmacy: Effects against nociception, inflammation, and pyrexia in rats and mice, J. Ethnopharmacol., 127: 85– 90. 128.
Adil, A., Shahid, A., Zakia, B., (2024). Phytochemical Composition, Antioxidant and Antimicrobial Activities of Prosopis juliflora (Sw.) Dc. Grown in State of Azad Jammu and Kashmir, Pakistan. Pakistan Journal of Scientific & Industrial Research Series: Physical Sciences 67 (3).
Ahoyo, C. C., Deguenon, P. M., Dah-Nouvlessounon, D., Sina, H., Houehanou, T. D., Yaoitcha, A. S., ... & Houinato, M. R. (2019). Comparative in vitro antimicrobial effect of Sarcocephalus latifolius (Sm.) EA Bruce leaves and roots on foodborne pathogens. African Journal of Microbiology Research, 13(22), 357-368.
Ally-Charles, B., Ede, T., Basil, D., Karishma, J., Andrew H., Obena, V., Charlan, A., Jamain, H. (2024). Evaluation of The Total Antioxidant Capacity of Three Selected Plants in Guyana. International Journal of Pathogen Research 13 (6): 57-67.
Al-Ragib, A., Hossain, M. T., Hossain, J., and Jakaria, M. (2017). Antioxidant potential and cytotoxicity of Randia dumetorum Lam. leaf extract. Journal of Pharmacognosy and Phytotherapy. 9(9), pp. 138-145, DOI: 10.5897/JPP2016.0435 Article Number: 310811765924 ISSN 2141-2502. http://www.academicjournals.org/JPP
Apu, A. S., Muhit, M. A., Tareq, S.M., Pathan, A. H., Jamaluddin A. T. M., Ahmed, M. (2010). Antimicrobial Activity and Brine Shrimp Lethality Bioassay of the Leaves Extract of Dillenia indica Linn. Journal of Young Pharmacists, 2, 1, 50-53.
Badiaga M (2021). Etude ethnobotanique, phytochimique et activités biologiques de Nauclea latifolia Smith une plante médicinale africaine récoltée au Mali. Thèse de Doctorat unique. Université de Bamako, Mali et Université Blaise Pascal - Clermont-Ferrand II.
Baliyan S., Mukherjee, R., Priyadarshini, A., Vibhuti, A., Gupta, A., Pandey, R. P., Chang, C. (2022). Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. Molecules, 27 (4): 1326.
Brooks, R., Goldson-Barnaby, A., & Bailey, D. (2020). Nutritional and Medicinal Properties of Phyllanthus Acidus L. (Jimbilin). International Journal of Fruit Science, 20(sup3), S1706-S1710.
Carlos, C. A., Pauline, M. D., Durand, D. N., Haziz, S., Thierry, D. H., Alain, S. Y., Marcel, R. H. (2019). Comparative in vitro antimicrobial effect of Sarcocephalus latifolius (Sm.) EA Bruce. leaves and roots on foodborne pathogens. African Journal of Microbiology Research, 13(22), 357-368. https://doi.org/10.5897/ajmr2019.9062
Charles-Okhe, O., Odeniyi, M. A., Fakeye, T. O., Ogbole, O. O., Akinleye, T. E., & Adeniji, A. J. (2022). Cytotoxic activity of crude extracts and fractions of African peach (nauclea latifolia (Smith) stem bark on two cancer cell lines. Phytomedicine Plus, 2(1), 100212. https://doi.org/10.1016/j.phyplu.2021.100212
Da, F. L., Tindano, B., Zabre, G., Sakira, K., Bayala, B., Belemtougri, R. G., & Horlait, P. (2023). Effects of Sarcocephalus latifolius Fruits Extract on Paracetamol-Induced Liver Damage in Wistar Rats. Pharmacology & Pharmacy, 14(04), 112–122. https://doi.org/10.4236/pp.2023.144009.
Emmanuel, I. O., Adesina, A. B., Ohonmoimen, I. J., Azah, M., & Dar, F. A. (2021). In Vitro Anticancer Potential of Sacrocephalus Latifolius Leaves Extract and Its Fractions on Human MCF-7 and NCI-H460 Cancer Cell Lines. Asian Journal of Agriculture and Biology, 2021(3), 1–7. https://doi.org/10.35495/ajab.2020.10.520
Evbuomwan, I.O; Stephen Adeyemi,O; & Oluba,O.M. (2023). Indigenous Medicinal Plants Usedin Folk Medicine For Malaria Treatment in Kwara State, Nigeria: An Ethnobotanical Study. Medicine And Therapies, 23(1), 324
Ezenyi, I. C., Verma, V., Singh, S., Okhale, S. E., & Adzu, B. (2020). Ethnopharmacology-aided antiplasmodial evaluation of six selected plants used for malaria treatment in Nigeria. Journal of Ethnopharmacology, 254, 112694.
Filkpièrè Léonard, D. A., Mahamadou, B., Albert, S., Basile, T., Sékou, B., Balé, B. (2023). Effect of Sarcocephalus latifolius B. Aqueous Extract Fruits on Gentamicin-Induced Nephrotoxicity in Rats. Journal of Pharmacy and Pharmacology Research. 7: 12-19.
Ikpefan, E. O. (2019). Brine Shrimp Cytotoxic and Lemna minor Phytotoxic Evaluations of the Methanol Extracts of the Leaves, Stem and Root Barks of Sacrocephalus latifolius. Nigerian Journal of Basic and Applied Sciences, 27(2), 136.
Imah-Harry J.U., Omoleye I.S., Chidume C.C., & Ajayi K.O. (2025). Comparative Study of the Cytotoxicity, Antioxidant, and Antimicrobial Profile of Selected Medicinal Plants Used as Food Flavours in Eastern Nigeria. Funct. Food J 6(1):1168-182. https://ffnan.org/journals/journal-11
Imran, T. K., Muhammad N., Ullah, R., Imran, M. (2022). Triglyceride, Fatty Acid Profile, Antioxidant Characteristics, Vitamins, Lipid Oxidation and Induction Period of Ice Cream Produced from Cow and Buffalo Milk. Journal of Food Processing and Preservation 46 (12), e17278.
Journal of Modern Drug Discovery and Drug Delivery Research, pp. 1-7.
Kinshasa city (DR Congo) for their intimate hygiene and evaluation of the pH of derived recipes.
Krishnaraju, A. V., Rao, T. V. N., Sundararaju, D., Vanisree, M., Tsay, H. S., & Subbaraju, G. V. (2005). Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp Lethality Assay. International Journal of Applied Science and Engineering, 3(2), 125-130.
Lall, N., & Meyer, J. J. M. (1999). In vitro inhibition of the growth of Mycobacterium tuberculosis by extracts of South African medicinal plants. Journal of Ethnopharmacology, 66(3), 347-354.
Lawal, I. O., Uzokwe, N. E., I. Igboanugo, A. B., Adio, A. F., Awosan, E. A., Nwogwugwu, J. O., Faloye, B., Olatunji B. P., and Adesoga, A. A. (2010). Ethno medicinal information on collation and identification of some medicinal plants in Research Institutes of South-west Nigeria. African Journal of Pharmacy and Pharmacology, 4 (1). 001-007.
Matsuura, K., and Kurokawa, M. (2016), Metabolic Regulation of Apoptosis in Cancer: in International Review of Cell and Molecular Biology.
Mintah, S.O., Archer, M.-A., Asafo-Agyei, T., Ayertey, F., Jnr, P.A.-A., Boamah, D. and Barimah, K.B. (2022). Medicinal Plant Use in Ghana: Advancement and Challenges. American Journal of Plant Sciences, 13, 316-358. https://doi.org/10.4236/ajps.2022.133020
Muhammad, Z. I., Chinedu, I., and Arowora, K. A. (2023). Phytochemical constituents of ethanolic extracts of fruits of Acacaia nilotica and flowers of Calotropis procera. Inter. J. PharmaO2. 5(1): ISSN: 2582-4708, 8-13.
Ngbolua KN, Kabena NO, Lukoki LF, Mpiana PT, Ngombe KN, Fatiany RP, Baholy R (2014).
Obakiro, S. B., Kiprop, A., K’owino, I., Andima, M., Owor, R. O., Chacha, R. and Kigondu, E. (2022). Phytochemical, Cytotoxicity, and Antimycobacterial Activity Evaluation of Extracts and Compounds from the Stem Bark of Albizia coriaria Welw ex. Oliver. Evidence-Based Complementary and Alternative Medicine. Volume 2022, Article ID 7148511, 20 pages, https://doi.org/10.1155/2022/7148511
Obika O. I., Oyawaluja, A. A., Odukoya, O. A., Obika-Ndiri, N. A., Oiseoghaede, J. O. (2024). Evaluation of Sarcocephalus latifolius Afzel. ex R.Br. Rubiaceae on Reduction of Creatinine Level and Its Antioxidant In-Vitro. Journal of Fundamental and Applied Pharmaceutical Science, 5(1), 21-35.
Ovadje, P., Roma, A., Steckle, M., Nicoletti, L., Arnason, J.T. and Pandey, S. (2015). Advances in the Research and Development of Natural Health Products as Main Stream Cancer Therapeutics. Evidence-Based Complementary and Alternative Medicine, Volume 2015, Article ID 751348, 12 pages http://dx.doi.org/10.1155/2015/751348.
Owolabi C. O., Adeniran O. I., Joseph-Adekunle T. T., Bodunde, J. G., Olubode, O. O. (2020). Relevance of Edible Landscaping Concept in Food Security. Nigerian Journal of Horticultural Sciences. 25(2): 39-48.
Pale, S., Neteydji, S., Taiwe, G. S., Emegam, N. K., & Bum, E. N. (2022). Anticonvulsant effects of Cymbopogon giganteus extracts with possible effects on fully kindled seizures and anxiety in experimental rodent model of mesio-temporal epilepsy induced by pilocarpine. Journal of Ethnopharmacology, 286, 114863.
Patel, V.R., Patel, P. R., and Kajal, S.S. (2010). “Antioxidant activity of some selected medicinal plants in western region India,” Advances in Biological Research, 4, 23–26.
Pfeffer, C. M., and Singh, A. T. K. (2018). Apoptosis: A target for anticancer therapy. International Journal of Molecular Sciences; 19 (2) 448.
Phytochemical screening of some medicinal plants traditionally used by African Women in
Quinlan, M. B. (2022). Ethnomedicines: Traditions of Medicinal Knowledge. In book: A companion to medicinal anthropology. Pp 315-341
Rubio, C. P., Hernandez-Ruiz, J., Martinez-Subiela, S., Tvarijonaviiute, A., and Ceron, J. J. (2016). Spectrophotometric Assays for Total Antioxidant Capacity (TAC) In Dog Serum: An Update. BMC Veterinary Research 12: 166
Sharma, T., Tyagi, V., & Bansal, M. (2020). Determination of sun protection factor of vegetable and fruit extracts using UV–Visible spectroscopy: A green approach. Sustainable Chemistry and Pharmacy, 18, 100347.
Shukla, S. K. (2023). Conservation of medicinal plants: challenges and opportunities. Journal of Medicinal Botany, 7, 5–10. https://doi.org/10.25081/jmb.2023.v7.8437
Wise, L. A., Radin, R. G., Palmer J. R., Kumanyika S. K., Boggs, D. A., Rosenberg L. (2011). Intake of fruit, vegetables, and carotenoids in relation to risk of uterine leiomyomata. Am J Clin Nut. 94: 1620-31.
Yesufu, H.B., Khan, I. Z., Abdulrahman, F.I., Abatcha Y. Z. (2014). A survey of the phytochemical and antioxidant potential of the fruit extracts of Sarcocephalus latifolius (Smith) Bruce (Rubiaceae): J. Chem. Pharm. Res., 6(5):791-795.
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