The Phytochemical, Antioxidant and Antimicrobial Properties of Spiced and Unspiced Beverages From Sorghum Stem Sheath (Sorghum bicolor) and Brazilian Plume Leaf

The Phytochemical, Antioxidant and Antimicrobial Properties of Spiced and Unspiced Beverages From Sorghum Stem Sheath (Sorghum bicolor) and Brazilian Plume Leaf

Authors

  • D. P. Ogunmuyide Department of Food Science and Technology, Federal University of Technology Akure, Ondo State
  • J. O. Olorunnusi Department of Food Science and Technology, Federal University of Technology Akure, Ondo State
  • B. O. T. Ifesan Department of Food Science and Technology, Federal University of Technology Akure, Ondo State

DOI:

https://doi.org/10.51459/jostir.2025.1.1.01

Abstract

This study was carried out to investigate the ability of nonalcoholic, spiced and unspiced beverages produced from sorghum stem sheath and Brazilian plume leaf to serve as functional drink. The beverage blends were produced from Sorghum stem sheath and Brazilian plume leaf in the proportion of 100:0, 0:100 and 50:50 with or without spices respectively. The beverages were subjected to assays to examine their antioxidant, antimicrobial, phytochemical, alpha amylase and alpha glucosidase inhibitory properties. The antioxidant properties of the beverages were in the range of 69 %-80 % for 1,1-diphenyl-2 picryl hydroxyl free radical (DPPH), 23.00 mg/ml-58.00 mg/ml for ferric reducing antioxidant power (FRAP),0.664 mg/100ml-12.84 mg/100ml for vitamin C and 238.0 mg/ml- 288.0 mg/ml for anthocyanin content. The phytochemical content of the resultant beverages include flavonoid (6.12 mg/g-15.62 mg/g), alkaloid (1.52 mg/g -3.36 mg/g), tannin (0.77 mg/g-1.19 mg/g), oxalate (9.20 mg/g-19.71mg/g), saponin (2.52mg/g-8.78mg/g), anthraquinone (7.02 mg/g-11.82 mg/g), phenol (3.31 mg/g-8.21 mg/g) and terpenoid (29.14 mg/g-48.73 mg/g). The beverage blends were found to demonstrate very mild antimicrobial activities. The results showed that beverages exhibited good inhibitory potential against -amylase and -glucosidase enzymes which ranged from 60%-78% and 64%-77% respectively. The overall evaluation showed that spiced sorghum stem sheath-Justicia carnea beverages may be preferred to the unspiced beverage and could be considered as a functional drink.

References

Abiodun, O. B., Ifesan, B. T and Ifesan, B. O. T. (2022). Nutritional Composition of Spiced and Unspiced Beverage from Kiaat (Pterocarpus angolensis) Bark. Applied Tropical Agriculture. 27(2): 51 – 56.

Adedeji, T. O., Oluwalana I. B., and Ade-Omowaye B. I. O. (2013). Investigation on Antioxidant and Antinutritional Properties of Sorghum Stem Sheath-ginger Extract Based Non-alcoholic Beverage. International Journal of Food Science and Nutrition Engineering, 3(3): 28-34

Adedeji, T.O. (2020). Quality Evaluation of Sorghum bicolor Stem Sheath Enriched with Spondias mombin Extract.Archive of Food and Nutritional Science; 4: 012-019.

Adedeji, T.O., Gbadamosi, S.O and Taiwo, K.A. (2019). Comparative analysis on some quality attributes of Sorghum stem sheath-moringa instant and infused drinks. MOJ Food Process Technols.9 (1):18-26

Adetuyi AO, Pambang V, Oyetayo VO, Adetuyi FO. The Nutritive Value and Antimicrobial Property of Sorghum bicolor L. Stem (Poporo Flour Used as Food Colour Additive and its Infusion Drink. Am J Food Technol. 2007; 2: 79-86.

Adetuyi, A.O., Pambang, V., Oyetayo, V.O and Adetuyi, F.O.(2007).The Nutritive Value and Antimicrobial Property of Sorghum bicolor L. Stem (Poporo Flour Used as Food Colour Additive and its Infusion Drink. American Journal of Food Technology.2(2):79–86.

Adetuyi, A.O. (2004). Extraction and Spectroscopic Studies and Uses of Some Natural Dyes.Ph.D Thesis, Federal University of Technology, Akure, Nigeria. 95

Ahn, J., Grun, I.U and Mustapha, A.( 2007). Effects of plant extracts on microbial growth, colour change, and lipid oxidation in cooked beef. Food Micro. 24: 7-14.

Ajuru, M.G., Kpekot, K.A., Robinson, G.E., and Amutadi, M.C. (2022). Proximate and Phytochemical Analysis of the Leaves of Justicia carnea Lindi.andJusticia secunda Vahl and its Taxonomic Implications. Journal of Biomedicine and Biosensors 2(1): 1-12.

Ali, A and Gilani, A. H. (2007). Medicinal Value of Ginger with Focus on its Use inNausea and Vomiting of Pregnancy. Int. J. Food Properties10: 269–278.

Ali, K.E., Elsheikh, A.M, Ahmed, H.M., Osman, H.A., Hamza, N.B., Osman, M.G., and Daffalla, H.M. (2020). Phytochemical screening and antibacterial activities of Sorghum bicolor leaves derived from in vitro culture. GSC Biological and Pharmaceutical Sciences, 10(01): 065–072.

Amadi , B.A., Ibegbulem, C.O and Egbebu, A.C.(2006).Assessment of the effect of aqueous extract of Asimina triloba root on organ weights and liver function of albino rats. Int J Nat Appl Sci. 2: 79-81.

Ani, O.N., Udedi, S.C., Asogwa, K.K., Enemali, M.O., Onwelumadu, C.Mand Ikedife, K.S.(2020). Inhibitory Potential and Antidiabetic Activity of Leaf Extracts of Justicia carnea. International Journal of Biochemistry Research & Review. 29(6): 34-45.

Anthonia, O.C., Uroko, R.I., Njoku, O.U and Ezeanyika, L.U. (2019). Nutritive properties of aqueous extract Justicia carnea leaves and its effects on haematodogical and some biochemical indices of anaemia induced made wister albinorats. Biomedical Research. 30(4) 645-654.

AOAC. ( 2005). Official Methods of Analysis of the Association of Official Analytical chemists. 20th ed. Washington D.C.: s.n..

Asogwa, K.K., Udedi, S.C., Ani, O.N.,and Ekwualor, K.U. (2020). Evaluation of micronutrient composition and antioxidant effects of the extracts of the leaf of Justicia carnea. Sky Journal of Biochemistry Research . 7(2): 021 – 031.

Ayo-Omogie, H.N., Nwanna, E.M and Eguavoen, E.T.(2019). Evaluation of Antioxidant, Nutritional and Sensorial Properties of Novel Functional Beverage from Milk-cocoa Powder. African Journal of food science. 13(4): 83-91.

Bao, J., Cai, Y., Sun, M., Wang, G., & Corke, H. (2005). Anthocyanins, Flavonols, and Free Radical Scavenging Activity of Chinese Bayberry (Myrica rubra) Extracts and Their Color Properties and Stability. Journal of Agricultural and Food Chemistry. 53(6): 2327–2332.

Benderitter, M., Maupoli, V., Vergely, C., Dalloz, F. B., & Rochette, L. (1998). Studies by electron paramagnetic resonance of the important of iron in hydroxyl scavenging properties of ascorbic acid in plasma: effects of iron chelators.. Fundamental and clinical pharmacology, 12: 510-643.

Benderitter, M., Maupoli, V., Vergely, C., Dalloz, F., Briot, F. and Rochette, L. (1998). Studies by electron paramagnetic resonance of the importance of iron in the hydroxylscavenging properties of ascorbic acid in plasma: Effects of iron chelators. Fundamental of Clinical Pharmacology 12: 510 – 516.

Bernfeld P. Amylases, alpha and beta. In: Colowick SP, Kaplan NO. (Eds.), Methods in Enzymology, vol. 1. Academic Press, New York. 1955; 149–158

Bhandari, M.R., Jong-Amurakkum, N., Hong, J. and Kawabata, J. (2008). Alpha glucosidase and alpha amylase inhibitory activities of a Napalese medicinal herb pakhanbhed (Bergenia ciliate Haw). Food chemistry. 106:247-252.

Bolade, M.K., Oluwalana, I.B., Ojo, O. 2009. Commercial practice of roselle (Hibiscus sabdariffa L.) beverage production: Optimization of hot water extraction and sweetness level. World Journal of Agricultural Sciences, 5(1), 126-131.

Borokini, F. B., Komolafe, O. Y., and Adesuyi, A. T. (2019). Comparative Study onPhysiochemical Parameters of Functional Beverages Formulated From Sorghum bicolor Stem Sheath And Hibiscus sabdariffa. Journal of Chemical Society of Nigeria. 44( 6): 1113 -1120.

Brunner, J. H. (1984). Direct spectrophotometer determination of saponin.Analytical Chemistry. 34: 1324-1326.

Clinical and Laboratory Standard Institute CLSI. (2006). Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standards. 9th ed. Clinical and Laboratory Standards Institute document 2006a M2-9. Clinical and Laboratory Inst., 940, West Valley Road, Suite 1400, Wayne, Pa., 19087–1898 U.S.A.

Dada, A.A., Ifesan, B.O.T., &Fashakin, J.F. (2013). Antimicrobial and antioxidant properties of selected local spices used in “Kunun”beverage in Nigeria. Acta Scientiarum Polonorum Technologia Alimentaria, 12(4):373-378.

Day, R.A and Underwood, A.L. (1986). Qualitative Analysis.5th Edn., Prentice Hall Publication, Englewood Cliffs: 710.

Doyon, M and J. A. Labrecqu (2008). Functional Foods: A Conceptual Definition. British Food Journal, 110 (11): 1133-1149.

Egbere, O.J., Anuonye, J.C., Chollom, P.F and Okpara, P.V.(2007). Effects of Some Preservation Techniques on the Quality and Storage Stability of Zobo Drink (A Nigerian, non–alcoholic beverage from Hibiscus sabdariffa). Journal of Food Technology. 5(3):225–228.

Eskin, N and Tamir S (2006). Dictionary of Nutraceuticals and Functional Foods. Boca Raton, FL: Taylor and Francis Group/CRC Press.

Falode, J.A., Dada, O.P, Ajigbayi, O.I., Ajayi, M.M., Yusuf, A.O., Adeoye, B.O. and Bai, B.I. (2022).Antioxidant and antidiabetic properties, and high-performance liquid chromatography (HPCL) Finger point of Justicia carnea leaf extracts. Journal of pure and Applied sciences. 7(3): 51-76.

Fasoyiro, S.B. (2014). The value and utilization of spice plants in tropical Africa. J. Agricultural and Food Information 15: 109-120.

Faturoti, A.O., Ifesan, B.O.T., Awolu, O.O and Olaleye, T.M. (2023).Antioxidant and Physicochemical Properties of Local Beverage From Blends of Germinated Pumpkin Seed (Cucurbita maxima), Ginger and Zobo calyx. Proceedings: Agricultural Revolution for Sustainable Livelihood .11:516-526.

Gond, G. Annu, V. and Oshin, P.(2023). Development and Analysis of ascorbic acid and anthoayanin of Hibiscus sabdariffa tea blended with spices during storage. The pharma innovation journal. 12(6): 3451-3454.

Gyamfi, M. A., Yonamine, M and Aaniya, Y. (1999). Free scavenging action off medicinal herbs from Ghana.. General Pharmacology. 32: 661-667.

Harborne, I.B. (1973). Phytochemical methods: A guide to modern techniques of plant analysis. 2nd Edition, Chapman and Hall, New York, 88-185.

Ifesan B.O.T., Siripongvutikorn S., Hutadilok-Towatana, N. and Voravuthikunchai, S.P. (2009). Evaluation of the ability of Eleutherine americana crude extract as natural food additive in cooked pork. J. Food Sci. 74, 353-357.

Ifesan, B.O.T. and Olorunsola. E.A. (2018).Nutritional Composition and Antioxidant Properties of Instant Beverage Infusion from Blends of Moringa Leaf (Moringa oleifera), Zobo Calyx (Hibiscus sabdariffa) and Lemon Grass (Cymbopogon citrates). 13 (2): 1-2

Khoo, H.E., Azrina, A., Sou, T.T and See, M.L. (2017). Anthocyanidins and Anthocyanins: Coloured pigments as food, pharmaceutical ingredients and potential health benefits. Food Nutrition Research. 61(1):1361779.

Kim, K. Y., Nam, K. A., Kurihara, H., & Kim, S. M. (2008). Potent α-glucosidase inhibitors purified from the red alga Grateloupia elliptica. Phytochemistry. 69(16): 2820–2825.

Kotler, P., Keller, K. Marketing Management, First Edition, Pearson, Boston. (2016).

Li, J., Hwang, I. C., Chen, X., Park, H. J. Effects of chitosan coating on Curcumin loaded nano-emulsion: Study on stability and in vitro digestibility, Food Hydrocolloids (2016) 60; 138-147

Malik, V.S, Popkin, B.M, Bray, G.A. Despres, J.P, Wille, W.C and Hu, F.B. (2010). Sugar-Sweetened Beverages and Risk of Metabolic Syndrome and Type 2 Diabetes: A Meta-Analysis. Diabetes Care. 33(11): 2477-2483.

Mollet, B and Lacroix, C. (2007). Where biology and technology meet for better nutrition and health. Current Opinion in Biotechnology 18:154-155.

Nabavi, S.F., Di Lorenzo, A., Izadi, M., Sobarzo-Sanchez, E., Daglia, M and Nabavi, S.M. (2016). Antibacterial effects of cinnamon; from farm to food, cosmetic and pharmaceutical industries. Nutrition and Medicinal Journal, 7(3):7729-7748.

Ndife, J. (2016). Functional foods: Basics, Ingredients and Applications (1st ed.). Kaduna: Amotes Link Services and Publishers, 78-80.

Ndife, J., Alozie, E.N., Amaechi, C and Onwuzuruike, U.A.(2020). Development and comparative evaluation of spice-mix seasoinings from functional leaves of Uda, (Xylopia aethiopica), Nchanwu (Ocimum gratissimum) and Uziza (Piper guineense). FUTO Journal series. 6(2): 174-189.

Nur, S.M. Wan., M.D., Hahill, R.A and Fatimah, S. (2020). Effect of storage temperatures on physicochemical, phytochemicals and antioxidant properties of water melon juice. Metabolites. 12: (1) 75.

Nwafor, O.E and Ogiehor, I.S. (2003) Microbial stability of meat preserved by Hurdle Technology: Proc.Niger.Inst.Food Sci. Technol. 74-76.

Oboh, G.O., Akomolafe, T.L and Adetuyi O.A. (2010). Inhibition of Cyclophosphamide– induced Oxidative Stress in Brain by one Dietary Inclusion of Red Dye extracts from Sorghum (Sorghum bicolor) stem. J. Medicine and Foods. 13(95):1–5.

Okechukwu-Ezike, N.C and Oly-Alawuba, N.M. (2020). Assessment of the Phytochemical contents and antioxidants Accillos of some herbs and spices in south-Eastern Nigeria. Research J. Food and Nation. 4(3): 1-5

Olugbenga, O.O., Simeon, O.A., Samuel, A.A., Olugbemiga, S.F., Clement, O.O., Taiwo, O.G. (2022). Proximate composition, phytochemical profile, antioxidant, antidiabetic and anti-inflammatory properties of Justicia carnea leaf powder black sea J. Agriculture 5(4): 415-425.

Oluwalana , I., Ade–Omowaye, B.I.O and Adedeji, T.O.(2013). Nutritional Composition of a Non– Alcoholic Beverage Spiced with Zingiber officinale Extract Produced from Sorghum bicolor Stem sheath. International Journal of Food Science and Nutrition Engineering. 3(3):21–27.

Onyeabo, C., Achi, N. K., Ekeleme-Egedigwe, C. A., Ebere, C. U., and Okoro, C. K. (2017).Haematological and biochemical studieson Justicia carnea leaves extract in phenylhydrazine induced-anemia in albino rats. Acta Sci. Pol. Technol. Aliment. 16 (2): 217–230.

Otles, S.,and Cagindi , O.(2012). Safety Considerations of Nutraceuticals and Functional Foods. In: McElhatton A, Sobral PJA, Editors. Novel Technologies in Food Science. New York, N.Y.: Springer.121–136.

Oyaizu, M. (1986). Studies on products of browning reaction: Antioxidative activity of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition 44: 307 – 315.

Ozen, A.E, Pons, A and Tur, J.A. (2012). Worldwide Consumption of Functional Foods: A Systematic Review, Nutr. Rev.70:472–481.

Phan, T.T., Wang, L., See, P., Grayer, R.J., Chan, S.Y and Lee, S.T.(2001). Phenolic Compounds of Chromolaena odorata Protect Cultured Skin Cells from Oxidative Damage : Implication for Cutatenous Wound Healing. Biol. Pharm. Bull. 24(12):1373-1379.

Ping, X., Lin, C. and Yeufei, W.(2019). Effect of storage tune on antioxidant activity and inhibition on -amylase and -glucosidase of white tea.Food sci. Nutrition. 7(2): 636-644.

Pulido, R., Bravo, L. and Saura-Calixto, F.(2000). Antidiabetic Activities of Dietary Phenols are determined by a Modified Ferric Reducing/ntioxidant Power Assay. Journal of Agric. Chemistry 48:3396-3402.

Rai, B and Anand, S.C. (2008). Serum and Salivary Vitamin C in Periodontal Disease: Adv. Med.Dent. Sci. 2(2): 26.

Salman S., Peter R., Steven M., Muhammad M., Mayank A., Syed M.H., Shahran S., Muhammad R., Syed G. and Wissam I.K. (2023). Plant Based Diet and Its Effect on Cardiovascular Disease. International Journal of Environmental Research and Public Health. 20 (4): 3337.

Shahidi, F and Naczk, M. (2004). Phenolics in Foods and Nutraceuticals.CRC Press.

Singh, R.P., Murthy, C.K.N. and Jayaprakasha, G.K. (2002). Studies on the antioxidant activity of pomegranate (Punicagranatum) peel and seed extracts using in vitro methods. Journal of Agricultural and Food Chemistry 50: 81 – 86.

Singleton, V. L., Orthofer, R and Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent.Methods in Enzymology.152–178.

Soladoye, M.O and Chukwuma, E.C. (2012). Quantitative phytochemical profile of the leaves of Cissus populnea Guill. & Perr. (Vitaceae) – an important medicinal plant in central Nigeria. Archives of Applied Science Research. 4 (1):200-206

Sonam, K.S and Guleria, S. (2017). Synergetic Antioxidant Activity of Natural Products. Ann Pharmacol Pharm. 2(8):1086.

Sowonola, O.A, Akintunde,T.Y and Adedeji, F.( 2005). Influence of Malting on the Nutritional Characteristics of Kunnu–zaki. African J Biotechnol. 5: 1-12.

Taylor, J.R and Emmeanbux, M.N. (2021). Molecular diversity and Health benefits of carbohydrates from cereals and pulses. 87(4): 263-271.

Tiburski, J.H., Rosenthal, A., Deliza, R., De Oliveira, Godoy, R.L., and Pacheco, S. (2011). Nutritional properties of yellow Mombin (Spondias mombin L.) pulp. Food Res Int. 44: 2326-2331.

Udedi, S.C., Ani, O.N., Asogwa, K.K., Maduji, F.C andOkafor, C.N. (2020). In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea. Int. J. Biochem. Res. & Review. 29(4): 48-60.

Ukpabi-Ugo, J.C., Ndukwe,P.A and Iwuoha, A.G. (2019). Hepatoprotective Effect of Methanol Extract of Justicia carnea Leaves on Carbon Tetrachloride-Intoxicated Albino Rats. Biochemistry & Analytical Biochemistry.8: 381.

Ukpabi-Ugo, J.C., Uhuo, E.N., Alaebo, P.O. and Ekwere, A.K. (2020). Effects of meternal leaves extract of justicia carnea on blood glucose level and lipid profile in alloxan-induced diabetic albino rate. Journal of pharmaceutical and Allied Sciences.17(2).

Uroko, R.I., Egba, N.K., Achi, O.N.. Uchenna, A., Agbafor, O., Ngwu, P and Nweje-Anyalowu, C.E. (2017). Effects of Aqueous Extracts of Palm Fruits (Elaeis guineensis) on Liver Function Indices of Male Wistar Albino Rats. Res. J. Med. Plants. 11: 148-159.

Wheeler, E.L and Ferrel, R.E. (1971).A Method for Phytic Acid Determination in Wheat and Wheat Fractions. Cereal Chemistry. 48: 312-320.

Wood, C. and Pittler, M.H. (2000). Comparison of efficacy of ginger with various anti-motions sickness drugs. British J. Anaesthesia. 84(3): 367 – 71.

Xinyan, B. Joseph, L and Christiani, J.H. (2017).Spices in the management of diabetes mellitus. Food Chemistry. 217:281-293.

Zaborowska, Z., Przygoński K. and Bilska, A.( 2012). Antioxidative effect of thyme (Thymus vulgaris) in sunflower oil. Acta Sci. Pol., Technol. Aliment. 11 (3), 283-291.

Downloads

Published

2025-07-30

How to Cite

Ogunmuyide, D. P., Olorunnusi, J. O., & Ifesan, B. O. T. (2025). The Phytochemical, Antioxidant and Antimicrobial Properties of Spiced and Unspiced Beverages From Sorghum Stem Sheath (Sorghum bicolor) and Brazilian Plume Leaf. Journal of Science, Technology and Innovation Research, 1(1). https://doi.org/10.51459/jostir.2025.1.1.01

Issue

Section

Articles
Loading...